Patientenkolloquium 2019
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Project TP02





Investigators:
Prof. Dr. Henrik Dommisch
Department of Periodontology, Operative and Preventive Dentistry
Center of Dento- Maxillo- Facial Medicine
Medical Faculty
University of Bonn


Prof. Dr. Dr. Søren Jepsen, M.S.
Department of Periodontology, Operative and Preventive Dentistry
Center of Dento- Maxillo- Facial Medicine
Medical Faculty
University of Bonn



First Funding Period

The role of antimicrobial peptides during development and progression of periodontal diseases

Summary
Within the oral cavity multiple bacterial species interact with epithelia, yet the oral cavity maintains healthy homeostasis. Antimicrobial peptides such as human beta-defensins (hBDs) are mainly responsible for maintaining oral health by providing a strong chemical barrier and thereby fight pathogenic bacteria. The hypothesis to be tested in this proposal is that gingival keratinocytes and gingival fibroblasts recognize oral bacteria differently and respond by cell specific gene expression patterns. This work will focus on distinctive genes involved in innate immune responses such as hBDs and C-C-chemokine-20, but also inflammatory mediators such as Interleukin-4, -6, -8, and -10. After exposure to oral bacteria (Streptococcus gordonii and Porphyromonas gingivalis), the gene expression will be analyzed by real-time PCR technology. This proposal will also investigate differential regulations of immune responses by specific cell surface receptors, such as pattern recognition receptors (Toll-like receptors, TLR-2 and TLR-4) and protease-activated receptors (PAR-1 and PAR-2), by utilizing RNA interference (RNAi) technology. Identifying differences in cell responses and communication may help to understand how gingival inflammation and especially periodontitis develops.


Poster



Second Funding Period

Antimicrobial peptides in gingival inflammation and periodontal healing

Summary
The evaluation of responses of gingival epithelial cells (GECs) and fibroblasts (HGFs) to the commensal Streptococcus gordonii and the pathogenic Porphyromonas gingivalis revealed that the commensal modified the antimicrobial host responses to the pathogen. We formulated a research agenda to further analyze gingival responses to oral bacteria in vitro and in vivo. (1) In GECs and HGFs, we aim to evaluate the antimicrobial response to various bacterial virulence factors, such as lipopolysaccharides, proteases etc.. Here, we propose that specific combinations of virulence factors are required to individually initiate antimicrobial peptide (AMPs) expression. (2) In addition, in vivo expression of AMPs will be investigated during early gingival inflammation. For this purpose, we aim to perform an experimental gingivitis study, a non-invasive human model of acute inflammation and its resolution. (3) Moreover, we aim to elucidate the expression profile of AMPs during periodontal healing in patients with advanced chronic periodontitis that undergo periodontal therapy. In both in vivo projects, the individual microbiological composition of the biofilm will be longitudinally analyzed. In the future, improved knowledge about the regulation of AMPs during host-bacteria-interactions is a pre-requisite for novel antimicrobial prevention and treatment strategies, involving AMPs.