Patientenkolloquium 2019
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Project TP07





Investigators:
Prof. Dr. med. Werner Götz

Department of Orthodontics
Center of Dento-Maxillo-Facial Medicine
Medical Faculty
University of Bonn


Dr. med. dent. Birgit Rath-Deschner
Department of Orthodontics
Center of Dento-Maxillo-Facial Medicine
Medical Faculty
University of Bonn



First Funding Period

Role of the insulin-like growth factor-system in the periodontium under different stress conditions

Summary
Mechanical, inflammatory and hypoxic stimuli are important clinical stress factors in the periodontium. There are indications that the insulin-like growth factor (IGF) system plays an important role in modulating these stress reactions on the cellular level. The aim of the project is to analyze the involvement of the IGF system, especially in the cells of the periodontal ligament (PDL), in vitro and in vivo. The reactions of IGF system components, the involvement of certain IGF receptor activated signal transduction molecules and crossroads to other stress related pathways in PDL cells will be investigated on gene expression and protein levels after defined mechanical stimulation. Hypoxic and inflammatory stress reactions will be determined with special reference to integrin mediated responses and p53. In further experiments, IGF-I and IGF-II will be applied to PDL cells stressed by mechanics, hypoxia and inflammation. In rat models of experimentally induced tooth movement and induced occlusal hyper- and hypofunction the response of the IGF system will be analyzed in vivo. The appearance and distribution of IGF system components and important molecules involved in the signal pathways will be studied by means of immunohistochemistry and ins-situ hybridization.

Poster


Secound Funding Period

Oxidative stress and interactions with biomechanical loading on cell metabolism of periodontal tissues

Summary
In the periodontal ligament (PDL) and alveolar bone oxidative stress seems to be correlated to mechanical, hypoxic and inflammatory influences and involved in the pathogenesis of occlusal overloading, inflammation and systemic disease effects. Oxidative stress is characterized by a disequilibrium in redox signaling and can lead to cellular dysfunctions and cell death. For the detection of oxidative stress, biomarkers like reactive oxygen species (ROS), enzyme systems and antioxidants can be used. Such markers will be investigated in human PDL cells and alveolar osteoblasts after mechanical, hypoxic and LPS stimulation using gene expression, biochemical and immunocytochemical methods. The activation of ROS related signal transduction pathways and transcription factors will also be evaluated. Cells will be stimulated with ROS to investigate their behavior under oxidative stress conditions. Supernatants from these experiments will be used to treat osteoclast precursors as a model system for ROS mediated bone loss in periodontitis. In healthy probands and patients suffering from periodontal diseases, gingival non-invasive oxygen measurements will be undertaken and gingival biopsies investigated for the expression of different ROS and antioxidants giving new insights into the role of oxidative stress in periodontal physiology and disease.